pBABE BRG1

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联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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pBABEBRG1载体基本信息

质粒名称: pBABE BRG1 
出品公司: 爱迪基因
目录编号: 1959
存储实验室: Robert Kingston

载体骨架基本信息
载体名称: pBABE-puro
载体抗性: 氨苄霉素
空载体大小: 5200 bp
完整质粒大小: 10200 bp
载体类型: 哺乳细胞表达,逆转录病毒
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: BRG1
物种来源: 人源
插入大小: 5000 bp
基因又名: BAF190a
标签/融合蛋白: flag (C terminal on insert)
克隆方法: 限制性内切酶
5' 克隆位点: SalI(未破坏)
3' 克隆位点: SalI (未破坏)
5' 测序引物 pBABE 5'

文献引用方法
材料和方法部分: pBABE BRG1 was a gift from Robert Kingston (爱迪基因 plasmid # 1959)
参考文献部分: Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Genes Dev 2001 Mar 1;15(5):603-18. 10.1101/gad.872801 PubMed 11238380

pBABEBRG1载体图谱质粒图谱和多克隆位点信息

pBABE BRG1质粒图谱

pBABEBRG1载体简介

pBABEBRG1载体序列

LOCUS       pBABE BRG1               10182 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pBABE BRG1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Sif S, Saurin AJ, Imbalzano AN, Kingston RE
  TITLE     Purification and characterization of mSin3A-containing Brg1 and hBrm
            chromatin remodeling complexes.
  JOURNAL   Genes Dev 2001 Mar 1;15(5):603-18.
  PUBMED    11238380
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pBABE BRG1|
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          59..155
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             5096..5119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5137..5157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5142..5471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5322..5457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5384..5403
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5481..6080
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5481..5500)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5977..5997
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6301..6770
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6900..6917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7071..7659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7151..7170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7819..8679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8442..8461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8680..8784)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             8815..9284
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    9349..9548
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9749..10165
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     10095..10117
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     10133..10149
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gtggtacgta ggaattcgcc agcacagtgg tcgacggtat cgataagctt gataactggt
       61 aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag
      121 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      181 tctaaaagct gctctagccc tcgacggcgc cggcggctcc tgcaggaggc cactgtctgc
      241 agctcccgtg aagatgtcca ctccagaccc acccctgggc ggaactcctc ggccaggtcc
      301 ttccccgggc cctggccctt cccctggagc catgctgggc cctagcccgg gtccctcgcc
      361 gggctccgcc cacagcatga tggggcccag cccagggccg ccctcagcag gacaccccat
      421 ccccacccag gggcctggag ggtaccctca ggacaacatg caccagatgc acaagcccat
      481 ggagtccatg catgagaagg gcatgtcgga cgacccgcgc tacaaccaga tgaaaggaat
      541 ggggatgcgg tcagggggcc atgctgggat ggggcccccg cccagcccca tggaccagca
      601 ctcccaaggt tacccctcgc ccctgggtgg ctctgagcat gcctctagtc cagttccagc
      661 cagtggcccg tcttcggggc cccagatgtc ttccgggcca ggaggtgccc cgctggatgg
      721 tgctgacccc caggccttgg ggcagcagaa ccggggccca accccattta accagaacca
      781 gctgcaccag ctcagagctc agatcatggc ctacaagatg ctggccaggg ggcagcccct
      841 ccccgaccac ctgcagatgg cggtgcaggg caagcggccg atgcccggga tgcagcagca
      901 gatgccaacg ctacctccac cctcggtgtc cgcaacagga cccggccctg gccctggccc
      961 tggccccggc ccgggtcccg gcccggcacc tccaaattac agcaggcctc atggtatggg
     1021 agggcccaac atgcctcccc caggaccctc gggcgtgccc cccgggatgc caggccagcc
     1081 tcctggaggg cctcccaagc cctggcctga aggacccatg gcgaatgctg ctgcccccac
     1141 gagcacccct cagaagctga ttcccccgca gccaacgggc cgcccttccc ccgcgccccc
     1201 tgccgtccca cccgccgcct cgcccgtgat gccaccgcag acccagtccc ccgggcagcc
     1261 ggcccagccc gcgcccatgg tgccactgca ccagaagcag agccgcatca cccccatcca
     1321 gaagccgcgg ggcctcgacc ctgtggagat cctgcaggag cgcgagtaca ggctgcaggc
     1381 tcgcatcgca caccgaattc aggaacttga aaaccttccc gggtccctgg ccggggattt
     1441 gcgaaccaaa gcgaccattg agctcaaggc cctcaggctg ctgaacttcc agaggcagct
     1501 gcgccaggag gtggtggtgt gcatgcggag ggacacagcg ctggagacag ccctcaatgc
     1561 taaggcctac aagcgcagca agcgccagtc cctgcgcgag gcccgcatca ctgagaagct
     1621 ggagaagcag cagaagatcg agcaggagcg caagcgccgg cagaagcacc aggaatacct
     1681 caatagcatt ctccagcatg ccaaggattt caaggaatat cacagatccg tcacaggcaa
     1741 aatccagaag ctgaccaagg cagtggccac gtaccatgcc aacacggagc gggagcagaa
     1801 gaaagagaac gagcggatcg agaaggagcg catgcggagg ctcatggctg aagatgagga
     1861 ggggtaccgc aagctcatcg accagaagaa ggacaagcgc ctggcctacc tcttgcagca
     1921 gacagacgag tacgtggcta acctcacgga gctggtgcgg cagcacaagg ctgcccaggt
     1981 cgccaaggag aaaaagaaga aaaagaaaaa gaagaaggca gaaaatgcag aaggacagac
     2041 gcctgccatt gggccggatg gcgagcctct ggacgagacc agccagatga gcgacctccc
     2101 ggtgaaggtg atccacgtgg agagtgggaa gatcctcaca ggcacagatg cccccaaagc
     2161 cgggcagctg gaggcctggc tcgagatgaa cccggggtat gaagtagctc cgaggtctga
     2221 tagtgaagaa agtggctcag aagaagagga agaggaggag gaggaagagc agccgcaggc
     2281 agcacagcct cccaccctgc ccgtggagga gaagaagaag attccagatc cagacagcga
     2341 tgacgtctct gaggtggacg cgcggcacat cattgagaat gccaagcaag atgtcgatga
     2401 tgaatatggc gtgtcccagg cccttgcacg tggcctgcag tcctactatg ccgtggccca
     2461 tgctgtcact gagagagtgg acaagcagtc agcgcttatg gtcaatggtg tcctcaaaca
     2521 gtaccagatc aaaggtttgg agtggctggt gtccctgtac aacaacaacc tgaacggcat
     2581 cctggccgac gagatgggcc tggggaagac catccagacc atcgcgctca tcacgtacct
     2641 catggagcac aaacgcatca atgggccctt cctcatcatc gtgcctctct caacgctgtc
     2701 caactgggcg tacgagtttg acaagtgggc cccctccgtg gtgaaggtgt cttacaaggg
     2761 atccccagca gcaagacggg cctttgtccc ccagctccgg agtgggaagt tcaacgtctt
     2821 gctgacgacg tacgagtaca tcatcaaaga caagcacatc ctcgccaaga tccgttggaa
     2881 gtacatgatt gtggacgaag gtcaccgcat gaagaaccac cactgcaagc tgacgcaggt
     2941 gctcaacacg cactatgtgg caccccgccg cctgctgctg acgggcacac cgctgcagaa
     3001 caagcttccc gagctctggg cgctgctcaa cttcctgctg cccaccatct tcaagagctg
     3061 cagcaccttc gagcagtggt ttaacgcacc ctttgccatg accggggaaa aggtggacct
     3121 gaatgaggag gaaaccattc tcatcatccg gcgtctccac aaagtgctgc ggcccttctt
     3181 gctccgacga ctcaagaagg aagtcgaggc ccagttgccc gaaaaggtgg agtacgtcat
     3241 caagtgcgac atgtctgcgc tgcagcgagt gctctaccgc cacatgcagg ccaagggcgt
     3301 gctgctgact gatggctccg agaaggacaa gaagggcaaa ggcggcacca agaccctgat
     3361 gaacaccatc atgcagctgc ggaagatctg caaccacccc tacatgttcc agcacatcga
     3421 ggagtccttt tccgagcact tggggttcac tggcggcatt gtccaagggc tggacctgta
     3481 ccgagcctcg ggtaaatttg agcttcttga tagaattctt cccaaactcc gagcaaccaa
     3541 ccacaaagtg ctgctgttct gccaaatgac ctccctcatg accatcatgg aagattactt
     3601 tgcgtatcgc ggctttaaat acctcaggct tgatggaacc acgaaggcgg aggaccgggg
     3661 catgctgctg aaaaccttca acgagcccgg ctctgagtac ttcatcttcc tgctcagcac
     3721 ccgggctggg gggctcggcc tgaacctcca gtcggcagac actgtgatca tttttgacag
     3781 cgactggaat cctcaccagg acctgcaagc gcaggaccga gcccaccgca tcgggcagca
     3841 gaacgaggtg cgtgtgctcc gcctctgcac cgtcaacagc gtggaggaga agatcctagc
     3901 tgcagccaag tacaagctca acgtggacca gaaggtgatc caggccggca tgttcgacca
     3961 gaagtcctcc agccatgagc ggcgcgcctt cctgcaggcc atcctggagc acgaggagca
     4021 ggatgaggag gaagacaagg tgcccgacga cgagaccgtc aaccagatga tcgcccggca
     4081 cgaggaggag tttgatctgt tcatgcgcat ggacctggac cgcaggcgcg aggaggcccg
     4141 caaccccaag cggaagccgc gcctcatgga ggaggacgag ctcccctcgt ggatcatcaa
     4201 ggacgacgcg gaggtggagc ggctgacctg tgaggaggag gaggagaaga tgttcggccg
     4261 tggctcccgc caccgcaagg aggtggacta cagcgactca ctgacggaga agcagtggct
     4321 caaggccatc gaggagggca cgctggagga gatcgaagag gaggtccggc agaagaaatc
     4381 atcacggaag cgcaagcgag acagcgacgc cggctcctcc accccgacca ccagcacccg
     4441 cagccgcgac aaggacgacg agagcaagaa gcagaagaag cgcgggcggc cgcctgccga
     4501 gaaactctcc cctaacccac ccaacctcac caagaagatg aagaagattg tggatgccgt
     4561 gatcaagtac aaggacagca gcagtggacg tcagctcagc gaggtcttca tccagctgcc
     4621 ctcgcgaaag gagctgcccg agtactacga gctcatccgc aagcccgtgg acttcaagaa
     4681 gataaaggag cgcattcgca accacaagta ccgcagcctc aacgacctag agaaggacgt
     4741 catgctcctg tgccagaacg cacagacctt caacctggag ggctccctga tctatgaaga
     4801 ctccatcgtc ttgcagtcgg tcttcaccag cgtgcggcag aaaatcgaga aggaggatga
     4861 cagtgaaggc gaggagagtg aggaggagga agagggcgag gaggaaggct ccgaatccga
     4921 atctcggtcc gtcaaagtga agatcaagct tggccggaag gagaaggcac aggaccagct
     4981 gaagggcggc cggcggcggc cgagccgagg gtcccgagcc aagccggtcg tgagtgacga
     5041 tgacagtgag gaggaacaag aggaggaccg ctcaggaagt ggcagcgaag aagacgacta
     5101 caaggacgac gatgacaaat gataagtcga ccctgtggaa tgtgtgtcag ttagggtgtg
     5161 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5221 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5281 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     5341 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5401 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5461 gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5521 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5581 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5641 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5701 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5761 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5821 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5881 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5941 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6001 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     6061 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     6121 cacgacccca tgcatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     6181 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     6241 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     6301 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     6361 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     6421 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     6481 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     6541 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     6601 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     6661 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     6721 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
     6781 gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat
     6841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7501 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7741 tcaaaaagga tcttcaccta gatccttttg cggccgcaaa tcaatctaaa gtatatatga
     7801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7861 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7921 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7981 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8041 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8101 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8161 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8221 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8281 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8341 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8401 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cagcctgaat
     8821 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     8881 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     8941 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     9001 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     9061 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     9121 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     9181 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     9241 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     9301 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggcaa gctggccagc
     9361 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
     9421 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
     9481 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
     9541 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
     9601 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
     9661 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
     9721 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     9781 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     9841 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     9901 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     9961 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
    10021 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
    10081 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
    10141 agccctcact ccttctctag gcgccggccg gatcccagtg tg
//

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